Analytical

Liberty Blue Microwave Peptide Synthesizer

Liberty Blue Microwave Peptide Synthesizer Peptide synthesis made fast and efficient

 

CEM is transforming the way chemists perform peptide synthesis once again with the introduction of the Liberty Blue™ Microwave Peptide Synthesizer. More than just a facelift, the Liberty Blue System has been designed for maximized speed and effi ciency. With a 4-minute cycle time and a 90% reduction in solvent consumption, no other peptide synthesizer on the market can even come close to the performance of the Liberty Blue. Unparalleled peptide quality, greater fl exibility, and improved reliability make Liberty Blue the system of choice for all your peptide synthesis needs.

 

Remarkably fast

  • Rapid reagent addition
  • Optimized synthesis methods
  • >80% time reduction compared to 1st generation microwave synthesizers
  • Difficult peptides such as A-beta in hours rather than days

 

The 4-minute cycle time of the Liberty Blue System is not limited to easily synthesized peptides. These methods can be used to routinely synthesize a wide range of peptides. Using the standard Liberty Blue methods, A-beta 1-42 was prepared in less than 4 hours in 68% crude purity.

 

  • 4-minute cycle time
  • 90% solvent reduction

 

Maximum efficiency

  • Up to 90% solvent reduction
  • Less reagent usage
  • No priming required
  • Judicious use of solvent for washing
  • Accurate and precise reagent addition

 

Benefits of microwave control

Liberty Blue offers several advantages over alternative heating methods. The microwave energy can be controlled for each step, and the reaction temperature can be carefully selected depending on the specific peptide and/or reagents used. CEM is the only microwave provider than can apply the microwave energy to the entire process. Microwave irradiation heats the reaction from the inside rather than the outside making it possible to rapidly change the temperature from one stage to the

next. This can be especially important when synthesizing peptides containing temperature-sensitive amino acids, such as phosphopeptides, or peptides containing difficult to couple residues such as Aib. The synthesis of the double Aib containing peptide below was performed with the coupling of the second Aib residue at both 75 °C and at 95 °C with all of the rest of the conditions the same. The coupling at 75 °C resulted in only 44% crude purity with a significant Aib deletion while the 95 °C

gave 90% crude purity.

 

  • Flexibility
  • Cleavage option
  • Amino Acid Bottles
  • Reaction Vessels
  • Fiber Optic
  • Microwave
  • Solvent Resistant Composite Covers
  • Waste Level Detection
  • Compact Footprint
  • LED Lights

 

Flex-Add Technology

Flex-Add Technology Liberty Blue’s patent-pending Flex-Add Technology is a new approach to reagent addition that utilizes pressure to measure reagent volume. The Flex-Add Technology is both accurate and precise over a large volume range, allowing for both small and large scale syntheses to be performed on the system seamlessly.

  • Simplicity
  • Reliability
  • Liberty Blue Software

 

Options for the Liberty Blue System

UV Monitoring

Resin Loader

Cleavage option

Increased Purity of Diffi cult Sequences

 

The Microwave Advantage

Microwave energy directly couples at the molecular level with polar and ionic species. The movement of the molecules trying to align themselves with the electric field of the microwave causes motion, and thus, produces heat. However, it is this transfer of kinetic energy that makes all of the difference in microwave peptide synthesis. The constant realignment of the peptide chain in the microwave field accelerates the reaction and helps to keep it from aggregating, significantly improving results of the synthesis.

  • License included with each system allowing the use of microwave energy for both the coupling and deprotection steps
  • Fastest cycle times available
  • Unparalleled peptide purity and yield compared to conventional synthesis
  • Significantly reduces purification time and waste
  • Access to peptides impossible to synthesize under conventional conditions

 

 

SPECIFICATIONS

Synthesis Scale                         0.025 – 5 mmol

Reaction Vessel Sizes               10-, 30- and 125-mL standard

Chemistry                                  Fmoc only

Activation Method                     In situ, standard activators

Amino Acid Reagents                20, plus 7 additional positions

External Bottle Positions          2, plus 7 additional positions

Temperature Measurement       In situ fi ber-optic

Fluid Measurement                    Flex-Add – amino acids, activator, activator base Timed delivery – Main wash, Deprotection

Agitation                                     Programmable inert gas bubbling

Waste Container                        1 L reservoir with overfl ow detection

Controller                                    Laptop with wireless option standard

Optional Features                      UV monitor

                                                     Resin Loader for high throughput

                                                     Cleavage module

Reports                                       Printable .pdf fi le for each method created. Log created during each run of every step performed

Power                                          120V/60Hz or 240V/50Hz

Dimensions                                20”W x 18”D x 30”H

                                                    (51 cm x 46 cm x 76 cm)

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